State-controlled radio Mozambique reported that the money would be used to help cut new HIV infections in the country to 2.4 per a thousand people from the current 3.6.
Tuberculosis (TB), malaria and HIV/AIDS are identified as the diseases responsible for high mortality rates in the country.
The Global Fund, a grouping of governments, civil society and the private sector which invests some $4 billion a year into programmes to fight infectious diseases, will complement the PEPFAR, an initiative of the US President's Emergency Plan for AIDS Relief.
The Maputo-based broadcaster quoted Health Minister Nazira Abdula as saying the government hopes to improve viral control methods in order to prevent mainly vertical transmission in pregnant women and children, who are groups deemed vulnerable to the disease.
"The government plans to reduce the incidence of tuberculosis from 551 to 276 per 100,000 inhabitants by 2020, and in this programme we will also work with other partners mainly in the prevention, education and basically treatment component", Abdulla was quoted as saying.
TB is a bacterial infection which kills more people each year in Mozambique than any other infectious disease, according to the national health authorities.